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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Effect of sex-specific differences on function of induced hepatocyte-like cells generated from male and female mouse embryonic fibroblasts

Fig. 3

Expression of hepatocyte-specific genes and proteins in miHep. (A) Hepatocyte-specific markers, (a) mAlb, (b) mAfp, (c) mAat, (d) mTrf, (e) mEcad, and (f) mVim analyzed by real-time quantitative PCR. MEFs and liver were used as a negative and positive control for each gene, respectively. *Above the bars represent a statistically significant difference at p < 0.05. Male liver tissue was used as a positive control for hepatocyte-specific genes. (B) Comparison of expression of hepatocyte-specific proteins (Alb, Aat, and Ecad) between (Bb) male and (Bd) female miHeps. MEFs were used as a negative control for each protein. Green and red fluorescence indicate positive expression of each protein, and blue fluorescence indicates DAPI staining of the nucleus. Scale bar = 100 μm. The data represent the relative quantification (RQ) ± min and max RQ values

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Stem Cell Research & Therapy

ISSN: 1757-6512