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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Effect of sex-specific differences on function of induced hepatocyte-like cells generated from male and female mouse embryonic fibroblasts

Fig. 4

Xenobiotic metabolizing Cyps analysis and albumin and urea secretion in miHep. miHeps treated for 3 days with specific Cyp inducers, 3-methylcholanthrene (3-MC, +) for Cyp1a1 (a), Cyp 1a2 (b), Cyp 3a11 (c) and Cyp 3a13 (d); Rifampicin (RIF, ++) for Cyp 2a5 (B); and dexamethasone (DEX, +++) for Cyp 2d22 (C). Relative mRNA levels were normalized to male miHeps, in the absence of chemicals. The data represent the relative quantification (RQ) ± min and max RQ values. *Above the bars represent a statistically significant difference at p < 0.05. (D) Secretion levels of (a) albumin and (b) urea by ELISA in each cell culture supernatant of male and female miHeps cultured for 2 and 3 days. Fresh culture medium for miHeps was used as control. *Above the bars represent a statistically significant difference at p < 0.05. # indicates statistically significant difference with all groups. The data represent means ± SD

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