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Fig. 7 | Stem Cell Research & Therapy

Fig. 7

From: Effect of sex-specific differences on function of induced hepatocyte-like cells generated from male and female mouse embryonic fibroblasts

Fig. 7

Liver function in acute liver injury model transplanted with miHep. (A) Serum of mice was collected 4 days after CCl4 injection and analyzed for aspartate aminotransferase (AST, a), alanine aminotransferase (ALT, b), alkaline phosphatase (ALP, c), albumin (ALB, d), total bilirubin (T-BIL, e), and total protein (TP, f) using a Hitachi clinical analyzer 7180 (Hitachi. Ltd., Tokyo, Japan). Before transplantation, 1–2 × 106 miHeps labeled with Pkh26 (Red fluorescence) and transfused into spleen of female and male mouse (5 mice/group). PBS group used as manipulated control (n = 5). * or # above the bars represent a statistically significant difference by LSD or Tukey HSD post hoc test, respectively at p < 0.05. The data represent means ± SD. (B) Section of liver transplanted with miHep stained with hematoxylin/eosin (H&E) or Masson’s trichrome staining for detection of collagen fiber. A health liver; b, liver at 1 day after CCl4 injection; c, liver at 3 days after transfusion of PBS; d liver at 3 days after transfusion of miHep. (C) Section of liver transplanted with male miHep stained by Pkh26. Red signals found around blood vessel. Blue (DAPI) signals meant counter staining to confirm nucleus of cell. White dot squares enlarged as shown below. White asterisks indicate cells with a bipolar nucleus originated from male miHep

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