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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: MSI-1436 improves EMS adipose derived progenitor stem cells in the course of adipogenic differentiation through modulation of ER stress, apoptosis, and oxidative stress

Fig. 1

Evaluation of morphology, proliferation rate, and adipogenic differentiation of investigated cells. Visualization of cells cultured in standard growth medium (undifferentiated—ND) and cells which underwent adipogenic differentiation (AD) (a). BrdU assay for cell proliferation (b). Accumulation of lipid droplets was visualized with Oil Red O staining and cells were pictured with confocal microscope (c). Transcript levels of C/EBPalpha (d), PPARγ (e), Lep (f), and Adipoq (g) were determined with RT-qPCR. ELISAs revealed the extracellular levels of PPARγ (h), leptin (i), and adiponectin (j). Relative changes in the expression of genes related to adipogenesis and metabolic regulation: Akt1 (k), Akt2 (l), AHSG (m), and SHBG (n) established by RT-PCR. Results expressed as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001. Magnification × 100; scale bar, 250 μm

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