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Table 2 Fatty acid composition of the FFA lipid class in equine ASCs healthy, EMS, adipogenic treated, and untreated cells

From: MSI-1436 improves EMS adipose derived progenitor stem cells in the course of adipogenic differentiation through modulation of ER stress, apoptosis, and oxidative stress

FFAs Retention time (min) Mass spectra match [%] EqASCs_HE ND EqASCs_EMS ND EqASCs_HE AD EqASCs_EMS AD EqASCs_EMS+MSI-1436 AD
Content (μg/2 × 106 cells)
Tetradecanoic acid, methyl ester; ME. C14:0 24.24 95 2.64 ± 0.04 4.89 ± 0.17*** 3.15 ± 0.25**;### 2.745 ± 0.10*;### 3.24 ± 0.04##
Pentadecanoic acid, methyl ester; ME. C15:0 26.92 95 0.735 ± 0.10 0.21 ± 0.08* 0.81 ± 0.08# 1.05 ± 0.08### 1.8 ± 0.42***;###
Hexadecanoic acid, methyl ester; ME. C16:0 29.5 92 40.23 ± 0.59 68.325 ± 0.27*** 28.47 ± 0.17***;### 24.96 ± 0.21***;### 30.195 ± 0.31***;###
9-Hexadecenoic acid, methyl ester, (E)-; ME. C16:1N(9E) 30.7 91 23.745 ± 0.02 3.165 ± 0.23*** 2.235 ± 0.10***;### 4.725 ± 0.10***;### 2.19 ± 0.08###
Octadecanoic acid, methyl ester; ME. C18:0 34.22 96 36.75 ± 0.46 52.26 ± 0.17*** 19.125 ± 1.5***;### 13.695 ± 0.10***;### 17.685 ± 0.10**;###
9-Octadecenoic acid (Z)-, methyl ester; ME. C18:1N(9Z) 34.8 93 1.275 ± 0.06 7.85 ± 0.27*** 19.125 ± 1.5***;### 8.82 ± 0.04*** 8.835 ± 0.06***
9-Octadecenoic acid (E)-, methyl ester; ME. C18:1N(9E) 34.9 92 2145 ± 0.148 17.085 ± 0.36*** 5.115 ± 0.10***;### 2.145 ± 0.15***;### 1.545 ± 0.19***;##
9,11-Octadecadienoic acid, methyl ester, (9Z,11Z); ME. C18:2N(9Z,11Z) 36.6 90 1.185 ± 0.02 1.38 ± 0.04*** 1.365 ± 0.02** 1.5 ± 0.04*** 1.845 ± 0.02***;###
6,9,12-Octadecadienoic acid, methyl ester (all Z); ME. C18:3N(6Z, 9Z, 12Z) 34.29 94 21.225 ± 0.06 1.71 ± 0.25*** 1.59 ± 0.08*** 5.385 ± 0.06***;### 0.495 ± 0.14***;###
  1. *Different from Healthy ASCs (*p < 0.05, **p < 0.01, ***p < 0.001)
  2. #Different from EMS cells ASCs