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Table 2 Fatty acid composition of the FFA lipid class in equine ASCs healthy, EMS, adipogenic treated, and untreated cells

From: MSI-1436 improves EMS adipose derived progenitor stem cells in the course of adipogenic differentiation through modulation of ER stress, apoptosis, and oxidative stress

FFAs

Retention time (min)

Mass spectra match [%]

EqASCs_HE ND

EqASCs_EMS ND

EqASCs_HE AD

EqASCs_EMS AD

EqASCs_EMS+MSI-1436 AD

Content (μg/2 × 106 cells)

Tetradecanoic acid, methyl ester; ME. C14:0

24.24

95

2.64 ± 0.04

4.89 ± 0.17***

3.15 ± 0.25**;###

2.745 ± 0.10*;###

3.24 ± 0.04##

Pentadecanoic acid, methyl ester; ME. C15:0

26.92

95

0.735 ± 0.10

0.21 ± 0.08*

0.81 ± 0.08#

1.05 ± 0.08###

1.8 ± 0.42***;###

Hexadecanoic acid, methyl ester; ME. C16:0

29.5

92

40.23 ± 0.59

68.325 ± 0.27***

28.47 ± 0.17***;###

24.96 ± 0.21***;###

30.195 ± 0.31***;###

9-Hexadecenoic acid, methyl ester, (E)-; ME. C16:1N(9E)

30.7

91

23.745 ± 0.02

3.165 ± 0.23***

2.235 ± 0.10***;###

4.725 ± 0.10***;###

2.19 ± 0.08###

Octadecanoic acid, methyl ester; ME. C18:0

34.22

96

36.75 ± 0.46

52.26 ± 0.17***

19.125 ± 1.5***;###

13.695 ± 0.10***;###

17.685 ± 0.10**;###

9-Octadecenoic acid (Z)-, methyl ester; ME. C18:1N(9Z)

34.8

93

1.275 ± 0.06

7.85 ± 0.27***

19.125 ± 1.5***;###

8.82 ± 0.04***

8.835 ± 0.06***

9-Octadecenoic acid (E)-, methyl ester; ME. C18:1N(9E)

34.9

92

2145 ± 0.148

17.085 ± 0.36***

5.115 ± 0.10***;###

2.145 ± 0.15***;###

1.545 ± 0.19***;##

9,11-Octadecadienoic acid, methyl ester, (9Z,11Z); ME. C18:2N(9Z,11Z)

36.6

90

1.185 ± 0.02

1.38 ± 0.04***

1.365 ± 0.02**

1.5 ± 0.04***

1.845 ± 0.02***;###

6,9,12-Octadecadienoic acid, methyl ester (all Z); ME. C18:3N(6Z, 9Z, 12Z)

34.29

94

21.225 ± 0.06

1.71 ± 0.25***

1.59 ± 0.08***

5.385 ± 0.06***;###

0.495 ± 0.14***;###

  1. *Different from Healthy ASCs (*p < 0.05, **p < 0.01, ***p < 0.001)
  2. #Different from EMS cells ASCs