Fig. 2

Characterization of EpSlKCs co-cultured with DP cells. a Schematic representation and b light microscopy images of EpSlKCs in co-culture with dermal papilla (DP) cells and in the respective monoculture controls. c DNA quantification of EpSlKCs after 5, 9, and 13 days of culture (n = 5). d Representative images of immunofluorescence staining against the epithelial markers K14 and K10, K6, and K15 after 5 days in culture. e Immunostaining against K14 staining in EpSlKCs after 13 days in culture and the respective flow cytometry quantitative data (n = 3). DAPI was used as nuclear counterstaining. Data shown are mean ± SEM. Scale bars are 100 μm for b and 50 μm for d and e. ****p < 0.0001; ····p < 0.0001 vs. day 5; ^##p < 0.01, ^###p < 0.001, and ^####p < 0.0001 vs. day