Fig. 5

HF and SG induction in mice. a Representative hematoxylin and eosin (H&E) images of the area where EpSlKCs and DP cells, b DP cells alone, or c vehicle were transplanted (dashed lines indicating the wound area). Immunostaining of the recreated hair follicle (HF)- and sebaceous gland (SG)-like structures against the epithelial markers i.a K14, i.b K15 (dashed circle), or i.c both (white arrow). i.d Expression analysis of the differentiation marker K10 within the wound area (ii.a). H&E higher magnification image evidencing an apparently differentiated core (black arrowheads) within the formed structures, and the presence of small vessels around them (yellow arrowheads). ii.b Immunodetection of the fatty acid-binding protein 4 (FABP4) within the structures resembling SGs. Scale bars are 500 μm for a–c and 50 μm for i.a–i.d and ii.a and ii.b