Fig. 1
Osteogenic differentiation and oxidative stress level of HBMSCs co-cultured with HAMSCs, lncRNA-ANRIL expression in HBMSCs, and effects of lncRNA-ANRIL on the proliferation of HBMSCs. a Relative mRNA expression levels in HBMSCs were measured by RT-PCR analysis. b, c ROS and SOD levels in HBMSCs were measured using flow cytometry and xanthine oxidase assay kit. d LncRNA-ANRIL expression in HBMSCs was measured by RT-PCR analysis. e HBMSC proliferation was demonstrated by flow cytometry. Data are represented as mean ± standard error of the mean for all groups. *P < 0.05 and **P < 0.01. LPS: LPS + HBMSCs; HAMSCs, HAMSCs + LPS + HBMSCs; NC: HAMSCs + LPS + HBMSCsNC; ANRIL: HAMSCs + LPS + HBMSCsANRIL; shNC: HAMSCs + LPS + HBMSCsshNC; shANRIL: HAMSCs + LPS + HBMSCsshANRIL