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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Human amnion-derived mesenchymal stem cells promote osteogenic differentiation of lipopolysaccharide-induced human bone marrow mesenchymal stem cells via ANRIL/miR-125a/APC axis

Fig. 2

HAMSCs promoted the osteogenesis of LPS-induced HBMSCs via the downregulation LncRNA-ANRIL. a ALP staining and activity in HBMSCs. Scale bar, 100 μm. b Alizarin red staining and quantification in HBMSCs. Scale bar, 100 μm. c Relative mRNA expression levels in HBMSCs were measured by RT-PCR analysis. d Relative protein levels in HBMSCs were assessed by Western blot analysis. Data are represented as mean ± standard error of mean for all groups. *P < 0.05 and **P < 0.01. LPS: LPS + HBMSCs; HAMSCs: HAMSCs + LPS + HBMSCs; NC: HAMSCs + LPS + HBMSCsNC; ANRIL: HAMSCs + LPS + HBMSCsANRIL; shNC: HAMSCs + LPS + HBMSCsshNC; shANRIL: HAMSCs + LPS + HBMSCsshANRIL

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