Fig. 5
MiR-125a targeted APC, activated Wnt/β-catenin pathway, and alleviated osteogenesis. a The potential binding sites between APC and miR-125a predicted using biological software. b APC protein level was assessed by Western blot analysis in the NC, mimics, iNC, and inhibitor groups. c APC mRNA expression was measured by RT-PCR analysis in the NC, mimics, iNC, and inhibitor groups. NC: HAMSCs + LPS + HBMSCsmiR-125a NC; mimics: HAMSCs + LPS + HBMSCsmiR-125a mimics; iNC: HAMSCs + LPS + HBMSCsmiR-125a iNC; inhibitor: HAMSCs + LPS + HBMSCsmiR-125a inhibitor. d Luciferase reporter assay was used to validate the target in 293T cells. Relative Renilla luciferase activity was normalized to that of firefly luciferase. e APC protein level was assessed by Western blot analysis in the NC and APC groups. f Relative mRNA expression of β-catenin was measured by RT-PCR analysis in the NC and APC groups. g Immunofluorescence staining showed a β-catenin location in the NC and APC groups. Scale bar, 50 μm. h Relative protein levels were assessed by Western blot analysis in the NC and APC groups. i Relative mRNA expression levels were measured by RT-PCR analysis in the NC and APC groups. NC: HAMSCs + LPS + HBMSCsAPC NC; APC: HAMSCs + LPS + HBMSCsAPC. Data are represented as mean ± standard error of the mean for both groups. *P < 0.05 and **P < 0.01