Fig. 3

GDF-5 promotes EpSCs proliferation via Foxg1/cyclin D1 in vitro. Mouse EpSCs were infected with pAdEasy-Myc control adenovirus or pAdEasy-Foxg1. After the cells were transfected for 48 h with siRNA control plasmid or cyclin D1 siRNAs, mouse EpSCs were treated with 100 ng/ml GDF-5 for 24 h. a Fluorescence effects of pAdEasy-Myc and pAdEasy-Foxg1 infected mouse EpSCs (× 100 magnification). b Foxg1 protein expression level was analyzed by WB. Control: non-infected normal mouse EpSCs. c Three siRNAs were synthesized to inhibit cyclin D1 expression and quantification of WB. d Cell proliferation was measured by CCK-8. e The PCNA levels were analyzed by WB. The data are shown as the means ± SD of three independent experiments. **P < 0.01 vs. Control, ^^P < 0.01 vs. siControl, ^##P < 0.01 vs. the GDF-5 group