Fig. 4

GDF-5 promotes EpSCs proliferation via Foxg1/cyclin D1 in vivo. The groups including control, pAdEasy-Myc, cyclin D1 siRNA, GDF-5, pAdEasy-Foxg1 + GDF-5, and cyclin D1 siRNA + GDF-5. a After mouse EpSCs were labeled by BrdU, BrdU⁺PCNA⁺-positive EpSCs were analyzed in wound. BrdU⁺ and PCNA⁺ cells in the regenerated epidermis are shown at the same magnification. Bar = 50 μm. b BrdU⁺ and PCNA⁺ cell count was performed using Image Pro Plus in the regenerated epidermis. The data are presented as the means ± SD of three independent experiments; **P < 0.01 vs. the control group, ^##P < 0.01 vs. the GDF-5 group