Fig. 5
From: GDF-5 promotes epidermal stem cells proliferation via Foxg1-cyclin D1 signaling
GDF-5 regulates cyclin D1 protein and mRNA expression through Foxg1. Mouse EpSCs were infected with pAdEasy-Myc control adenovirus or pAdEasy-Foxg1 and the cells were treated with 100 ng/ml GDF-5 for 24 h. a Cyclin D1 mRNA expression. b Cyclin D1 protein expression. c, d Mouse EpSCs were transfected with pAdEasy-Myc control adenovirus or pAdEasy-Foxg1 or the luciferase reporter expression vectors or mutated pGL3-pF2 vector using Lipofectamine 2000. The data are presented as the means ± SD of three independent experiments; **P < 0.01 vs. the control, ##P < 0.01 vs. the GDF-5 group