Skip to main content
Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Infrapatellar fat pad-derived mesenchymal stem cell-based spheroids enhance their therapeutic efficacy to reverse synovitis and fat pad fibrosis

Fig. 1

IFP-MSC spheroid immunophenotypic profile and tripotentiality. a In 2D settings, Crude IFP-MSC show stable MSC-related phenotypic profile with high expression levels for CD44 and CD90 markers and increased donor-to-donor variability in CD10, CD146, and CXCR4 expression levels. b, c Upon seeding in gas-permeable culture plates, Crude IFP-MSC can generate multiple sized spheroids (100–300 μm) with enhanced expression of CD90, CXCR4, CD10, and NG2. CD146 and CD44 show variable expression levels between spheroids. d IFP-MSC spheroids can efficiently differentiate towards chondrogenic, osteogenic, and adipogenic lineages after specific induction in gas-permeable plates. Quantitative molecular profiling showed that differentiation-related markers in induced IFP-MSC spheroids were increased compared to non-induced cultures, indicating their mature status. All experiments (n = 3) were performed independently, and data are presented as mean ± SD (*p < 0.05)

Back to article page