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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Infrapatellar fat pad-derived mesenchymal stem cell-based spheroids enhance their therapeutic efficacy to reverse synovitis and fat pad fibrosis

Fig. 3

Immunophenotype and molecular profile of CD146-selected IFP-MSC spheroids. a Upon seeding in gas-permeable culture plates, CD146POS IFP-MSC spheroids show as expected higher expression levels of CD146 (451 ± 4 AU vs 241 ± 18 AU) and higher expression levels of NG2 (362 ± 178 AU vs 287 ± 22 AU), whereas CD146NEG IFP-MSC spheroids show higher expression levels of CD10 (253 ± 27 AU vs 164 ± 13 AU) and CXCR4 (227 ± 3 AU vs 140 ± 14 AU). b Molecular profiling of CD146POS versus CD146NEG IFP-MSC spheroids revealed that 55 out of 90 genes tested were higher expressed in CD146POS IFP-MSC spheroids with 12 genes being more than twofold higher (MCAM, HGF, ALPL, DLX5, NES, MMP13, CD200, COL1A1, FZD9, PDGFRB, HIC1). Genes tested were grouped in phenotype/function-related cohorts. c A total of 26 genes are shared between Crude and CD146POS spheroids, indicating similarities in their molecular signatures. However, upon TI or TIC induction, most of the immunoregulatory-related genes tested show upregulated expression levels in CD146POS spheroids. All experiments were performed independently (n = 3)

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Stem Cell Research & Therapy

ISSN: 1757-6512