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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Infrapatellar fat pad-derived mesenchymal stem cell-based spheroids enhance their therapeutic efficacy to reverse synovitis and fat pad fibrosis

Fig. 5

Immunomodulatory functionality of Crude IFP-MSC spheroids. a CFSE-labeled, PMA/IO-activated human PBMC showed a proliferation of ~ 91.2% for male PBMC donors and of ~ 89.9% for female PBMC donors, which was suppressed in a PBMC gender-dependent manner by co-culture with non-induced Crude or CD146-selected IFP-MSC spheroids (*p < 0.05, n = 3). b In both non-induced and TIC-induced SYN, non-induced Crude IFP-MSC spheroids can effectively form explant-cultures on synoviocyte monolayer whereas TIC induction of SYN significantly (*p < 0.05) upregulates inflammatory genes such as CD13 and PD-1. c SYN/IFP-MSC spheroid co-cultures resulted in significantly (*p < 0.05) higher secretion of immunomodulatory proteins (IL-6, IL-8, IP-10, MCP-1, MCP2, RANTES, TIMP-2) compared to TIC-induced SYN alone. All experiments were performed independently (n = 3)

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