Fig. 3

a Growth kinetics, b representative brightfield microscopic images, and c aggregate size distributions of hiPSCs seeded as single cells and cultured in either vertical-wheel or horizontal-blade bioreactors at agitation rates of 40 rpm, 60 rpm, and 80 rpm with no medium exchange (batch) for 6 days (scale bar = 200 μm). Cell samples were collected from n = 4 stirred suspension bioreactors at each condition. The P values were set at 0.05 and all graphs are presented with a ± standard error of the mean (SEM)