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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Radiation-induced toxicity in rectal epithelial stem cell contributes to acute radiation injury in rectum

Fig. 2

Pelvic irradiation reduces the regenerative response of LGR5+ stem cells in rectal crypts. a Schematic representation of the treatment schema for lineage tracing assay in Lgr5-eGFP-IRES-CreERT2; Rosa26-CAG-tdTomato mice. b Confocal microscopic images of the rectum section from Lgr5-eGFP-IRES-CreERT2; Rosa26-CAG-tdTomato mice. tdTomato (tdT)-positive cells are shown in red; Lgr5+ GFP+ cells are shown in green. Nuclei are stained with DAPI (blue). Marked expansion of tdT-positive red cells representing transit amplifying cells in crypt (regenerative crypt) were noted with un-irradiated rectal tissue compared to mice exposed to pelvic irradiation. c The number of regenerative crypt significantly decreased in irradiated mice rectum compared to un-irradiated control (n = 3; p < 0.007)

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Stem Cell Research & Therapy

ISSN: 1757-6512