Fig. 8

RNAseq analysis of Lgr5+ve and negative cells. LGR5+ and LGR5− cells derived from rectal crypts were sorted and sequenced to determine differential gene expression. a. Contour plot of flow-sorted LGR5+ (GFP+ve) and LGR5−ve rectal epithelial cells. b The heat map showed 728 genes differentially expressed genes between LGR5+ and LGR5− cells derived from rectal tissue. c List of stem cell marker exclusively expressed in rectum (p value adjusted cutoff of 0.05). These markers do not express in any other part of intestine. d Ingenuity Pathway Analysis show genes upregulated were principally associated with cell signaling, post-translation modification, and organ morphology network and to cell cycle, DNA replication, recombination, and repair