Fig. 1
From: Cholangiogenic potential of human deciduous pulp stem cell-converted hepatocyte-like cells
Transplanted donor SHED-Heps engraft without cell fusion in livers of recipient CCl4-treated mice. a A schema of SHED-Hep transplantation (SHED-HepT) into chronically CCl4-treated mice. Mice were intraperitoneally treated with CCl4 (1 mg/kg in olive oil, red arrowheads) twice a week for 8 weeks and administrated SHED-Heps (1.0 × 106/mouse) 4 weeks after CCl4 treatment. The mice were harvested 8 weeks after CCl4 treatment. b Representative images of in vivo kinetics of donor SHED-Heps were detected in CCl4-treated mice 24 h after SHED-HepT by DiR labeling. c–i Distribution of donor SHED-Heps was analyzed in the livers 4 weeks after the transplantation. Representative histogram of human leucocyte antigens A, B, and C (HLA-ABC) expression in the recipient whole liver cells (WLCs) by flow cytometric (FCM) assay. Area filled with red: target antibody-stained histograms; solid line: isotype-matched control-stained histograms. Number indicates averages of the positive rate (c). Representative images of HLA-ABC (d), hepatocyte paraffin 1 antigen (HepPar1; e), and human albumin (hALB; f) were detected by immunohistochemical analysis. Serum levels of hALB by enzyme-linked immunosorbent assay (ELISA). n = 5. nd, no detection. The graph bars represent the means ± standard error of mean (SEM) (g). Representative images of the expression of HepPar1 and hALB (h) and HepPar1 and mouse albumin (mALB, i) were detected by double immunofluorescent analysis. Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI). Merge: merged image. b, d–i: Cont, olive oil-treated mice; CCl4, CCl4-treated mice; SHED-Hep, SHED-Hep-transplanted CCl4-treated mice. d–f, h, i: Scale bars, 50 μm (d–f) and 10 μm (h, i)