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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Cholangiogenic potential of human deciduous pulp stem cell-converted hepatocyte-like cells

Fig. 4

SHED-Heps exhibit a potency into cholangiocyte-like cells. a A schema of induction of SHED-Heps into cholangiocyte-like cells (SHED-Chols). Generated SHED-Heps were cultured in William’s medium (WEM) with or without tumor necrosis factor alpha (TNFA +/−) for 4 days. Dex: dexamethasone; EGF: epidermal growth factor; FGF2: fibroblast growth factor 2; HGF: hepatocyte growth factor; IMDM: Iscove’s modified Dulbecco’s medium; ITS: insulin-transferrin-selenium premix solution; NCA: nicotinamide; OSM: oncostatin M. b Gene expression of hepatocyte nuclear factor6 (HNF6), SRY-box 9 (SOX9), KRT7, and KRT19 by RT-qPCR analysis. Results are shown as a ratio to human primary cholangiocyte (hChol = 1). n = 5 for all groups. *P < 0.05, ***P < 0.005. nd, no detection; ns, no significance. The graph bars represent the means ± SEM. c–e Representative images of SOX9, KRT7, and ALB expression were detected by immunofluorescent assay. Nuclei were stained with DAPI. Merge, merged image. Scale bars, 20 μm. b–e SHED-Chol−, TNFA-non-stimulated group; SHED-Chol+, TNFA-stimulated group

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