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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Ascorbic acid can promote the generation and expansion of neuroepithelial-like stem cells derived from hiPS/ES cells under chemically defined conditions through promoting collagen synthesis

Fig. 3

N2+AA medium can maintain the neural pluripotency of lt-NES cells. a Quantitative RT-PCR analysis of the expression level of NSC markers SOX1, PAX6, NESTIN, and SOX2 and pluripotency markers NANOG and OCT4 of P0 H9 neurosphere-derived lt-NES cells. b Quantitative RT-PCR analysis of the expression level of NSC markers SOX1, PAX6, NESTIN, and SOX2 and pluripotency markers NANOG and OCT4 of P0 UiPS neurosphere-derived lt-NES cells. n = 3 each. Data are expressed as means ± SD.*P < 0.05, **P < 0.01 vs. control. c Immunofluorescence for NSC markers SOX2 (red) and NESTIN (green) of P0 H9 & UiPS neurosphere-derived lt-NES cells. Scale bars = 100 μm. Nuclei were counterstained with DAPI (blue). d Quantification of SOX2- and NESTIN-positive cells of immunofluorescence of P0 H9 neurosphere-derived lt-NES cells. P, passage. e Quantification of SOX2- and NESTIN-positive cells of immunofluorescence of P0 UiPS neurosphere-derived lt-NES cells

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