Skip to main content
Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Ascorbic acid can promote the generation and expansion of neuroepithelial-like stem cells derived from hiPS/ES cells under chemically defined conditions through promoting collagen synthesis

Fig. 4

lt-NES cells can maintain long-term expansion and neural pluripotency using N2+AA medium. a Quantitative RT-PCR analysis of the expression level of NSC markers SOX1, PAX6, NESTIN, and SOX2 and pluripotency markers NANOG and OCT4 of P5 H9 neurosphere-derived lt-NES cells. b Quantitative RT-PCR analysis of the expression level of NSC markers SOX1, PAX6, NESTIN, and SOX2 and pluripotency markers NANOG and OCT4 of P10 H9 neurosphere-derived lt-NES cells. n = 3 each. Data are expressed as means ± SD.*P < 0.05, **P < 0.01 vs. control. c Immunofluorescence for NSC markers SOX2 (red) and NESTIN (green) of P5&P10 H9 neurosphere-derived lt-NES cells. Scale bars = 100 μm. Nuclei were counterstained with DAPI (blue). d Quantification of SOX2+ and NESTIN+ cells of immunofluorescence. P, passage. e Representative karyotypes of H9 neurosphere-derived lt-NES cells at passage 15

Back to article page

Stem Cell Research & Therapy

ISSN: 1757-6512