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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Ascorbic acid can promote the generation and expansion of neuroepithelial-like stem cells derived from hiPS/ES cells under chemically defined conditions through promoting collagen synthesis

Fig. 5

lt-NES cells differentiated into functional neurons. A Neurons and glia differentiated from P10 H9 neurosphere-derived lt-NES cells (a, c). Immunofluorescence for neuronal marker class III β-tubulin (green) and glia marker GFAP (red) of Neurons and glia (b, d). B Neurons and glia differentiated of P10 UiPS neurosphere-derived lt-NES cells (a, c). Immunofluorescence for neurons marker class III β-tubulin (green) and glia marker GFAP (red) of neurons and glia (b, d). Scale bars = 100 μm. Nuclei were counterstained with DAPI (blue). C Quantitative RT-PCR analysis of the expression level of neurons markers NeuN, Neurog, β-tubulin, the glutamatergic neuron marker vGlut1, the motor neuron marker HB9 and the dopaminergic neuron marker ALDH1A1, astrocyte marker GFAP, and the oligodendrocyte marker OLIG2. Transcript levels were normalized to undifferentiated lt-NES cells, n = 3 each. D Representative images of activity map of neurons differentiated from H9 neurosphere-derived lt-NES cells on the 24-electrode array. E Spikes of neurons differentiated from H9 neurosphere-derived lt-NES cells in 30 s

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