Skip to main content
Fig. 7 | Stem Cell Research & Therapy

Fig. 7

From: Ascorbic acid can promote the generation and expansion of neuroepithelial-like stem cells derived from hiPS/ES cells under chemically defined conditions through promoting collagen synthesis

Fig. 7

AA activates ERK signaling in a collagen synthesis-dependent manner. a The morphology of lt-NES cells treated with AA or alternative antioxidants vitamin B1 (Vb1) and reduced glutathione (GMEE). b Quantitative RT-PCR analysis of the relative expression of collagen genes Col1a1 and Col4a1. n = 3 each. Data are expressed as means ± SD.*P < 0.05, **P < 0.01 vs. control. c Collagen deposition after AA treatment by Western blot analyze. d Quantitative statistics of collagen deposition after AA treatment by Western blot analyze. e The morphology of lt-NES cells treated with AA and collagen synthesis inhibitor AzC. f The whole-cell extracts of N2 cultured (coated) and N2+AA cultured (AA) lt-NES cells were analyzed by Western blot with phospho-specific antibodies against ERK1/2 (pERK1/2) and total ERK1/2. GAPDH was used as a control. g ERK activation is defined as the ratio of p-ERK/total ERK. n = 3 each. Data are expressed as means ± SD.*P < 0.05, **P < 0.01 vs. control. h The morphology of lt-NES cells when treated with or without ERK inhibitor and ERK activator. Scale bars = 100 μm

Back to article page

Stem Cell Research & Therapy

ISSN: 1757-6512