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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Human foreskin-derived dermal stem/progenitor cell-conditioned medium combined with hyaluronic acid promotes extracellular matrix regeneration in diabetic wounds

Fig. 1

The clonogenic capacity and surface marker expression of hFDSPCs. The clonogenic capacity of hFDSPCs was examined using CFU assays. a hFDSPCs cultured at P1 to P3 were seeded in 6-well plates at a density of 50 cells/cm2, with cell clones observed after 7 days. b Colony formation numbers were counted; the data were plotted in graphs and analysed using Graph Pad Prism Software (n = 6). c The colony morphology was observed under an inverted microscope, bar = 1000 μm. d Immunophenotyping of hFDSPCs was characterised using flow cytometry analysis. hFDSPCs were analysed for expression of the following markers (n = 4): CD90 (97.53% ± 0.48%), CD44 (97.28% ± 0.52%), CD105 (22.57% ± 2.09%), CD34 (1.30% ± 0.75%), CD45 (0.73% ± 0.39%), CD29 (98.62% ± 1.18%), CD13 (98.06% ±0.26%), CD59 (98.80% ± 1.02%), CD31(2.07% ± 0.31%), and CD133 (0.88% ± 0.16%). Isotype-matching IgG-FITC and IgG-PE were used to determine nonspecific signals. Data are shown as means ± SD, **p < 0.01, ***p < 0.001

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