Fig. 1From: Human foreskin-derived dermal stem/progenitor cell-conditioned medium combined with hyaluronic acid promotes extracellular matrix regeneration in diabetic woundsThe clonogenic capacity and surface marker expression of hFDSPCs. The clonogenic capacity of hFDSPCs was examined using CFU assays. a hFDSPCs cultured at P1 to P3 were seeded in 6-well plates at a density of 50 cells/cm2, with cell clones observed after 7 days. b Colony formation numbers were counted; the data were plotted in graphs and analysed using Graph Pad Prism Software (n = 6). c The colony morphology was observed under an inverted microscope, bar = 1000 μm. d Immunophenotyping of hFDSPCs was characterised using flow cytometry analysis. hFDSPCs were analysed for expression of the following markers (n = 4): CD90 (97.53% ± 0.48%), CD44 (97.28% ± 0.52%), CD105 (22.57% ± 2.09%), CD34 (1.30% ± 0.75%), CD45 (0.73% ± 0.39%), CD29 (98.62% ± 1.18%), CD13 (98.06% ±0.26%), CD59 (98.80% ± 1.02%), CD31(2.07% ± 0.31%), and CD133 (0.88% ± 0.16%). Isotype-matching IgG-FITC and IgG-PE were used to determine nonspecific signals. Data are shown as means ± SD, **p < 0.01, ***p < 0.001Back to article page