Fig. 6

Effect of hFDSPC-CM on cell proliferation of HaCat and human fibroblasts in vitro. HaCat and human fibroblasts were treated with hFDSPC-CM or hADSC-CM at different concentrations (0, 5, 10, 20, 50, 100 μg/mL) for 72 h. Then, the CCK-8 assay was used to test the effects on HaCat (a) and human fibroblast (b) proliferation. Cell proliferative activity was assessed using the EdU incorporation assay. The cells were treated with PBS/hFDSPC-CM/hADSC-CM at a concentration of 20 μg/mL and separately and continuously cultured for 72 h. EdU-positive stained HaCat are shown (c), and the percentage of EdU-positive cells was measured from four randomly selected fields (d). EdU-positive stained human fibroblasts are shown (e), and the percentage of EdU-positive cells was measured from four randomly selected fields (f). Data are shown as means ± SD; bar = 100 μm; n = 4, *p < 0.05, **p < 0.01, ***p < 0.001