Fig. 1

miR-204-5p is downregulated during the osteogenic differentiation of hADSCs. a Flow cytometry was used to identify hADSCs by detecting specific marker proteins CD44, CD45, CD90, CD105 and HLA-DR. b Relative expression of miR-204-5p at each time point during the osteogenic differentiation of hADSCs detected by qRT-PCR. U6 was used as a reference gene. Relative mRNA levels of the osteogenic markers RUNX2, ALP and OCN at each time point during the osteogenic differentiation of hADSCs detected by qRT-PCR. GAPDH was used as an internal control gene. Data are presented as the mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.01 (n = 3 independent experiments)