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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: The miR-204-5p/FOXC1/GDF7 axis regulates the osteogenic differentiation of human adipose-derived stem cells via the AKT and p38 signalling pathways

Fig. 3

miR-204-5p overexpression inhibits the osteogenic differentiation of hADSCs. hADSCs were transfected with miR-204-5p mimics, miR-204-5p inhibitors or NC and cultured in PM or OM. a Left panel: Western blot of FOXC1 and GDF7 protein levels on day 14 after osteogenic induction. GAPDH was used as an internal control. Right panel: quantitative analysis of the protein levels. ALP staining (b) and ARS (c) on day 14 after osteogenic induction. Quantification of ARS was shown below. Data are presented as the mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001 (n = 3 independent experiments)

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