Fig. 5

FOXC1 reduces the suppressive effect of miR-204-5p on the osteogenic differentiation of hADSCs. hADSCs were co-transfected with miR-204-5p mimics and FOXC1 overexpression vector or empty vector or co-transfected with miR-204-5p inhibitors and si-FOXC1 and cultured in OM. ALP staining (a) and ARS (b) on day 14 after osteogenic induction. Quantification of ARS was shown below. c Predicted binding site of miR-204-5p in the 3′-UTR of FOXC1 mRNA. d Relative luciferase activity of cells in different groups. Data are presented as the mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001 (n = 3 independent experiments)