Fig. 6

SOD2 acted as the hub protein to associate oxidative phosphorylation (OXPHOS) and sirtuin signalling pathway. a The protein-protein interaction network of OXPHOS and the sirtuin signalling pathway. Protein circled with blue in OXPHOS indicated that they were also parts of the sirtuin signalling pathway. b The protein expression of all 8 antioxidant enzymes detected in the proteome data and SOD2 showed the most remarkable upregulation. c The combination chart showing the expression of SOD2 detected with qRT-PCR, western blotting and TMT methods. The upper columns represent the mRNA expression levels, the bottom columns represent the grey value of the western blotting and the line chart represents the TMT quantitative expression. The data are represented as the means ± standard deviation (SD). *p < 0.05; **p < 0.01; ****p < 0.0001. All data were collected at least in triplicate. d Representative bands of SOD2 protein. GAPDH was used as a loading control