Fig. 5

SKP-SC-EVs attenuated neuronal apoptosis after OGD. a Representative image of TUNEL staining of sensory neuron in different groups showing TUNEL positive (green) neurons in the control, OGD, OGD + EV, and OGD + EV + LY294002 groups, and DAPI (blue) labeled cell nuclei. Scale bar, 50 μm. b Histograms showing the percentage of TUNEL positive neurons in different groups. c Representative image of Western blot analysis for Bax and Bcl-2 expression in different groups. d, e Histograms showing decreased Bax expression level and increased Bcl-2 expression level after SKP-SC-EV treatment to OGD-injured sensory neurons, while the expression level of Bax and Bcl-2 was reversed by adding LY294002. n = 3; **p < 0.01, ***p < 0.001 compared with the control group; ^##p < 0.01, ^###p < 0.001, compared with the OGD group; ^§p < 0.05, ^§§p < 0.01, ^§§§p < 0.001 compared with the OGD + EV group