Fig. 1

Discovery of neuron restorative small molecule therapy, NNI-362. Phenotypic screen to measure both proliferative and neuron maturity capacity from commercial novel libraries (a); proliferation using Alamar Blue, n = 5 (b). Ratio of mature neuron (MAP2a,b⁺) to total cell numbers (Hoechst dye) from human neural progenitors at DIV12, n = 6–12. Structure and formula weight of NNI-362 (d). Oxidative cellular metabolism directly related to cell numbers was measured by fluorescence increase in Alamar Blue using emission filter 530/580 nm. Data were analyzed using Kruskal-Wallis with Group as factor. *p < 0.05 vs. controls