Fig. 2From: MiR-30c-5p regulates adventitial progenitor cells differentiation to vascular smooth muscle cells through targeting OPGMiR-30c-5p inhibits VSMC differentiation from adventitial Sca-1+ progenitor cells. Adventitial Sca-1+ progenitor cells were transfected with mimic control/miR-30c-5p mimic/inhibitor control/miR-30c-5p inhibitor 4–6 h prior to TGF-β1 induced differentiation. The cells were collected after another 72 h. The transfection efficiency of miR-30c-5p mimic and miR-30c-5p inhibitor was detected by RT-qPCR (a, b). The expression of SMαA, SM22α, SMMHC, and h1-caponin were detected by RT-qPCR (c, d). The expression of SMMHC and h1-caponin were detected by western blot (e, f). The expression of SMαA and h1-caponin were detected by immunofluorescence (g, h) (*P < 0.05, **P < 0.01, ns P ≥ 0.05)Back to article page