Fig. 4

OPG may be the target gene of miR-30c-5p during VSMC differentiation from adventitial Sca-1⁺ progenitor cells. Adventitial Sca-1⁺ progenitor cells were transfected with mimic control/miR-30c-5p mimic/inhibitor control/miR-30c-5p inhibitor 4–6 h prior to TGF-β1 induced differentiation. Cells were collected for protein chip sequencing after another 72 h. Overall regulation of 200 proteins by miR-30c-5p mimic and miR-30c-5p inhibitor (a, b). Representative proteins downregulated (fold change < 0.667) by miR-30c-5p mimic (c) and representative proteins upregulated (fold change > 1.500) by miR-30c-5p inhibitor (d). The proteins that were both downregulated (fold change < 0.800) by miR-30c-5p mimic and upregulated (fold change > 1.200) by miR-30c-5p inhibitor (e). Target site of miR-30c-5p on the mRNA 3′UTR of TNFRSF11B (alias of OPG) (f) (*P < 0.05, **P < 0.01, ns P ≥ 0.05)