Fig. 3

miR-455-3p in Exos-IL6 inhibits the expression of PIK3r1 in macrophages. a qPCR analysis of miR-455-3p and miR-424-5p expression levels in Exos-IL6 and Exos-NC. b, c Schematic diagram showing the putative miR-455-3p binding sites in PIK3r1. The sequences of wild-type PIK3r1 and mutant PIK3r1 are listed as well. Luciferase reporter gene assays were performed to measure the luciferase activity in macrophages. d, e Macrophages were transfected with miR-455-3p mimics, inhibitors, and their corresponding controls (mimics NC, inhibitors NC). qPCR assay and western blot were used to analyze the mRNA and protein levels of PIK3r1, respectively. Data are presented as the mean ± SEM (error bar) of at least three independent experiments. *p < 0.05, **p < 0.01, and ***p < 0.001