Fig. 5

miR-455-3p treatment of LPS-induced endotoxemia mice. a Experimental procedure in mice. b The inflammatory infiltration in mouse tissues was visualized by H&E staining and CD68 immunohistochemistry. c Biochemical assays of hepatic indexes: alanine aminotransferase (ALT), glutamine-oxaloacetate transaminase (AST), indirect bilirubin (IBIL), alkaline phosphatase (ALP), and total bilirubin (TBIL). d Flow cytometry was used to analyze the activation rate of CD14 + CD16+ or CCR2 + CD16+ monocytes in CD45+ T cells. e The levels of inflammatory mediators in the blood were measured by Luminex. G-CSF, granulocyte colony-stimulating factor; IL, interleukin; MCP-1, monocyte chemoattractant protein-1; MIP-1ɑ, macrophage inflammatory protein-1ɑ; IP-10, interferon induction protein-10. Data are presented as the mean ± SEM (error bars). N = 5 mice per group; *p < 0.05, **p < 0.01, and ***p < 0.001. NC, miR-455-3p agomir negative control; miR, miR-455-3p agomir