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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Disruption of histamine/H1R signaling pathway represses cardiac differentiation and maturation of human induced pluripotent stem cells

Fig. 1

Histamine 1 receptor is essential to heart development in zebrafish. a Kaplan-Meier survival analysis at different time points post-histamine (his), H1R antagonist (pyrilamine), and H2R antagonist (cimetidine) treatment. b Representative microscope images of the cardiac morphology in zebrafish embryos at 96 h post-fertilization (hpf). The red dotted circles show cardiac morphology, and the quantification of pericardial edema area is shown below (n = 15, respectively). Scale bar 200 μM. c The heart rates of zebrafish under histamine, pyrilamine, and cimetidine treatment at different time points (n = 15 in each group). d Representative cardiac images of cmlc2-mCherry transgenic zebrafish embryos. The white dotted circles show enlarged atrial, and the white arrow shows septal defects (the left panel). H&E staining images of zebrafish are shown in the right panel and the blue circles show heart morphology (n = 10). Scale bar 100 μM. e Representative transmission electron micrographs of zebrafish heart at 96 hpf after administration of histamine, pyrilamine, and cimetidine (n = 4). The white arrows show cardiac mitochondria, and MF represents myofibroblasts. Scale bar 1 μM. Data are expressed as the mean ± SEM. **p < 0.01, ***p < 0.001 vs control

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