Fig. 7

Histamine-preconditioned hiPSC-CMs improve cardiac function after MI. a Representative M-mode echocardiogram in sham and MI mice at 2 weeks after PBS- or histamine-treated hiPSC-CM implantation. b EF and FS of MI mice at 2 weeks after cell injection are shown. EF, ejection fraction; FS, fractional shortening. c Representative Masson’s trichrome staining of cardiac tissue obtained from mice administered with hiPSC-CMs. The quantitative results of infarcted size on day 14 post-MI or sham operation are shown. d Two weeks after cell implantation, the hiPSC-CMs were identified by CM-Dil (red) and cTnT, and the nucleus was stained with DAPI (blue) with the quantification of CM-Dil-positive cardiomyocytes in the injury area. Scale bar 100 μM. Data are expressed as the mean ± SEM. *p < 0.05, **p < 0.01 vs control (n = 7 for each group)