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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: MicroRNA-126 protects against vascular injury by promoting homing and maintaining stemness of late outgrowth endothelial progenitor cells

Fig. 2

The effect of miR-126 on CXCR4 expression on LOCs. Relative gene expression was determined for CXCR4 by real-time PCR at 0 h, 1 h, 2 h, 4 h, 6 h, 12 h, and 24 h after transfection with miR-126 inhibitor (a) or lenti–miR-126 (b). Western blots show the protein expression of CXCR4 at 0 h, 1 h, 2 h, 4 h, 6 h, 12 h, and 24 h after transfection with miR-126 inhibitor (c) or lenti–miR-126 (d). A laser scanning confocal microscope was used to confirm CXCR4 expression on the cell membrane of LOCs in different groups (e). Mean fluorescence intensity was calculated by average area intensities using image J (f). Data are presented as mean ± SD. *P < 0.05, **P < 0.01 vs respective control group; n ≥ 3. Scale bar = 100 μm

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