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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: MicroRNA-126 protects against vascular injury by promoting homing and maintaining stemness of late outgrowth endothelial progenitor cells

Fig. 5

Inhibitors of AKT/eNOS and ERK/VEGF pathways regulate CXCR4 expression and LOC migration. Protein (a, b) and mRNA (c) expression of CXCR4 measured by Western blotting and real-time PCR on LOCs pretreated with PD98059 (10 μM), GW644652 (10 μM), PX-316 (10 μM), or 7-nitroindazole (10 μM) for 1 h before infection with lenti-miR-126. Representative western blots of CXCR4 (a). Quantification of CXCR4 bands on LOCs pretreated with PD98059, GW644652, PX-316, or 7-nitroindazole (b). GADPH expression was used for protein level normalization. mRNA expression of CXCR4 (c). miR-126 induced LOC migration in response to SDF-1α (100 ng/mL) was dramatically inhibited by PD98059, GW644652, PX-316, or 7-nitroindazole (d cell count, e crystal violet staining). Data are presented as mean ± SD. ***P < 0.001 vs. lenti-miR-126 group; #P < 0.05 vs. DMSO group. n ≥ 3. Scale bar = 100 μm

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