Fig. 3
miR-142-3p modulated by exosomes was mediated by lncRNA-NEAT1. (a) Heat map of miRNAs differentially regulated by exosomeMIF in H2O2-treated cardiomyocytes. Red indicates up-regulation and blue indicates down-regulation. (b) qRT-PCR validation of differentially regulated miRNAs in H2O2-treated cardiomyocytes, with or without exosomeMIF pre-treatment. MSCs were transfected with siRNA against lncRNA-NEAT1 or control siRNA-NT, followed by MIF. The exosomes were then collected and added to cardiomyocytes treated with H2O2. Cardiomyocytes without treatment were used as a control. *P < 0.05, versus control; ▲P < 0.05 vs. H2O2; ○P < 0.05 vs. H2O2+exosomesMIF+siRNA-LncRNA-NEAT1. (c) Binding sites of lncRNA and miRNA. (d) Binding of lncRNA and miRNA was verified by dual-luciferase reporter demonstrating that miR-142-3p was a target gene of lncRNA-NEAT1. *P < 0.05, compared with control group. Cardiomyocytes were transfected with a mimic control (miR-NC mimic) or miR-142-3p mimic, followed by exosomeMIF, and then exposed to H2O2. Cardiomyocytes with or without exosomeMIF were subjected to H2O2. Untreated cardiomyocytes were used as a control. (e) Transfection efficiency was analyzed by qRT-PCR. *P < 0.05 versus miR-142-3p mimic. (f and g) Apoptosis was analyzed by FACS. Activities of (h) caspases 3/7 and (i) caspase 8 in cell lysates were measured by ELISA. Each column represents mean ± SD of three independent experiments. *P < 0.05 vs. control; ▲P < 0.05 vs. H2O2; ○P < 0.05 vs. H2O2+exosomeMIF+miR-142-3p mimic