Skip to main content
Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: LncRNA-NEAT1 from the competing endogenous RNA network promotes cardioprotective efficacy of mesenchymal stem cell-derived exosomes induced by macrophage migration inhibitory factor via the miR-142-3p/FOXO1 signaling pathway

Fig. 4

FOXO1 was a direct target of miR-142-3p. a Predicted binding sites between miR-142-3p and the FOXO1 3′-UTR. b Dual-luciferase assay was performed in cardiomyocytes after co-transfection with FOXO1 3′-UTR WT or mutant (MUT) plasmids, and miR-142-3p mimics. *P < 0.05 vs. control in the WT group. c, d Western blot analysis of FOXO1 and β-actin protein levels in cardiomyocytes transfected with mimic control or miR-142-3p mimic treated with the exosomeMIF, and subjected to H2O2. Cardiomyocytes with or without exosomeMIF were subjected to H2O2. Untreated cardiomyocytes were used as a control. *P < 0.05 vs. control; P < 0.05 vs. H2O2; P < 0.05 vs. H2O2+exosomeMIF miR-142-3p mimic. eg Cardiomyocytes were transfected with siRNA-FOXO1 or siRNA-NT. Untreated cardiomyocytes were used as a control. siRNA-mediated transfection efficiency was determined by qRT-PCR (e) and western blotting (f, g). Each column represents mean ± SD from three independent experiments. *P < 0.05 vs. siRNA-FOXO1

Back to article page