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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: HMOX1 is partly responsible for phenotypic and functional abnormalities in mesenchymal stem cells/stromal cells from placenta of preeclampsia (PE) patients

Fig. 3

Comparison of functional outcome between PE-DBMSCs and DBMSCs after H2O2 preconditioning. a Proliferation by MTS assay after cells were treated with 50 μM and 100 μM H2O2, for 72 h for pre-treatment (Pre) and in-treatment (In) (while performing the assays) testing conditions. b Adhesion measured after 2 h of the start of the experiment with pre- and in-treatment conditions using the xCELLigence real-time cell analyzer. c Migration assay measured after 24 h of the start of the experiments, while cells were preconditioned with H2O2 (pre- and in-treatment) using the xCELLigence real-time cell analyzer. d RT-PCR analysis of the adhesion molecules, IL-6, CXCR4, ICAM, and CXCL4, after preconditioning with H2O2. Data is represented as ΔΔ−2 (relative fold change) as compared with untreated control. Experiments were performed in triplicate using cells at passage #3 prepared from five individual placentae. Bars represent standard errors, *p ≤ 0.05

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