Fig. 3

Proliferative activity of native HATMSC supernatants in in vitro model of the chronic wound. a Metabolic activity of MSU-1.1, HSkMEC.2, and HaCaT cells cultured in serum-free medium and 1% O₂ was measured by MTT assay at days 0, 1, 2, and 3 following treatment with 10, 25, 50, and 100% of HATMSC supernatants. Untreated cells were used as a control. Data represents mean ± SEM, n = 3; *p < 0.05, **p < 0.01, ***p < 0.001. b Metabolic activity of MSU-1.1 cultured in the 50% supernatant from HATMSC1, primary HATMSC2, HATMSC2, HATMSC2D10, and HATMSC2F10 cells. Untreated cells were used as a control. Data represents mean ± SEM, n = 3