Fig. 3
Two-photon intravital microscopy of neutrophils and hUCB-MSC interactions in the liver of LysM-GFP+/− mice. a Representative images for each condition; (upper panel) red: hepatic microvasculature (Texas Red-Dextran signal); green: neutrophils (endogenous signal), (lower panel) red: CMTPX-labeled hUCB-MSCs (exogenous signal); green: neutrophils (endogenous signal). Conditions included control (Additional file 4: Video S1), LPS (LPS-only-treated; Additional file 5: Video S2), hUCB-MSCs + LPS (hUCB-MSCs-treated; Additional file 6: Video S3), and hUCB-MSCs (hUCB-MSCs only treated; Additional file 7: Video S4). The “guiding arrows” represent neutrophils in control and hUCB-MSC conditions. These data are representative of three independent experiments (original magnification, × 200; scale bar = 30 μm). b The graph shows the number of neutrophils per FOV (mm3) for each condition in a. Quantitative results indicate the average values ± SD of at least three independent experiments. The results were analyzed by a one-way ANOVA with Dunnett’s post hoc test. *p < 0.01 versus control, **p < 0.001 versus each condition