Fig. 4
Dynamic interactions between neutrophils and hUCB-MSCs in the inflamed liver of LysM-GFP+/− mice using two-photon intravital microscopy. Representative images of dynamic interactions; red: CMTPX-labeled hUCB-MSCs (exogenous signal); green: neutrophils (endogenous signal). a Neutrophils migrated toward the activated hUCB-MSCs upon LPS stimulation (Additional file 8: Video S5). b Neutrophils gathering toward hUCB-MSCs attempted to phagocytize hUCB-MSCs (Additional file 9: Video S6 and Additional file 10: S7). c Neutrophils that engulfed some hUCB-MSCs migrated from the original site to another site (Additional file 11: Video S8). d Neutrophils showed a significant increase in contact frequency with other adjacent neutrophils (Additional file 12: Video S9). e The graph shows the relative contact frequency between neutrophils per FOV (mm3) in the hUCB-MSC-treated condition (hUCB-MSC only versus hUCB-MSC + LPS). f The early phase (4 min) and late phase (68 min) of neutrophil swarming (Additional file 13: Video S10 and Additional file 14: S11). g The graph shows the number of neutrophils per FOV (mm3) during neutrophil swarming (early phase versus late phase). Quantitative results indicate the average values ± SD of at least three independent experiments. The results were analyzed by Mann–Whitney test. **p < 0.001 versus each condition. These data are representative of three independent experiments (original magnification, × 200; scale bar = 10 μm)