Fig. 5

UCMSC-CM restores the dysfunctions of diabetic vascular endothelial cells via M2 macrophages. The functions of high-glucose-induced HUVECs, which were treated with NCM (control) and UCMSC-CM or cocultured with LPS-treated macrophages (MΦ) or UCMSC-CM-treated MΦ, were determined by angiogenesis assays, migration assays, and chemotaxis assays. a Representative images of tube network in the angiogenesis assay. Images were taken at 24 h. Scale bar 200 μm. b Quantitative analysis of the total tube length using ImageJ software in the angiogenesis assay. c Representative images of the migration assay. Images were taken at 0 to 24 h. Scale bar 200 μm. d Quantitative analysis of the wound area in the migration assay. The scratch area was measured using the ImageJ software before and after incubation. e Quantitative analysis of chemotactic cell number in the chemotaxis assay. The chemotactic cells were fixed with 100% methanol and then stained with DAPI. The numbers of stained nuclei were counted in five high-power fields per well under fluorescence microscopy. n = 6, per group. *P < 0.05, **P < 0.01