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Fig. 8 | Stem Cell Research & Therapy

Fig. 8

From: Exosomes from bone marrow mesenchymal stem cells enhance fracture healing through the promotion of osteogenesis and angiogenesis in a rat model of nonunion

Fig. 8

The inhibition of BMP-2 decreased the Smad1 and RUNX2 protein levels in MC3T3-E1Cs stimulated with BMMSC-Exos. a Western blot analysis showed that even if BMMSC-Exos were applied, the expression levels of BMP-2, Smad1 and RUNX2 proteins were markedly increased after BMP-2 was inhibited. b The grey value ratios were quantified. c The immunocytochemistry analysis showed that applying BMMSC-Exos+BMP-2 inhibitor could not promote osteogenesis. d Gross scanning images of ALP and ARS staining of MC3T3-E1Cs (control group and BMMSC-Exos group), ALP staining at 14 days and ARS staining at 21 days. e Quantitative analysis of ALP and ARS activity was used to evaluate the effect of BMMSC-Exos on the osteogenic ability of MC3T3-E1Cs

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Stem Cell Research & Therapy

ISSN: 1757-6512