Fig. 8

Western blot and real-time PCR analysis of expression levels of potential targets of miR-144. A1–A3 Transfection of H9C2 cells with miR-144 mimics altered the effect of exosomes on expression of PTEN and Rac-1 in the context of hypoxia. ^###P < 0.001 vs. hypoxia; ^***P < 0.001 vs. hypoxia+Exo. B1–B3 Transfection of H9C2 cells with miR-144 mimics altered expression levels of PTEN and Rac-1 in hypoxic conditions. ^###P < 0.001 vs. normoxia; ^#P = 0.018 vs. normoxia; ^**P < 0.01 vs. hypoxia+ mimics control. C1–C3 Transfection of H9C2 cells with the miR-144 inhibitor altered expression levels of PTEN and Rac-1 in the induction hypoxia. ^#P = 0.038 vs. normoxia; ^##P < 0.01 vs. normoxia; ^**P < 0.01 vs. hypoxia+ inhibitor control. D1 Transfection of H9C2 cells with the miR-144 inhibitor had increased expression level of PTEN mRNA in hypoxic conditions. D2 Transfection of H9C2 cells with miR-144 mimics had decreased expression level of PTEN mRNA in hypoxic conditions. E1 The putative target sequence of PTEN 3′ UTR for miR-144-3p was from the TargetScan prediction software. E2 Activities of the luciferase in wild-type or mutant PTEN 3′ UTR reporter plasmids were detected by luciferase reporter assay after the transfection of cells with miR-144-3p or control. Exo, exosomes; PTEN, phosphatase and tensin homolog deleted on chromosome 10; Rac-1, Ras-related C3 botulinum toxin substrate 1, UTR, untranslated region. Statistics calculated based on the results of three repetitions of each experiment